Possible Answers a. lower the activation energy of the reaction catalyzed b. provide an alternate pathway for reaction which lowers the energy of the transition state c. are not consumed in the reaction d. usually display specificity toward a single reactant e. fail to influence the equilibrium point of the reaction 3. A protein was digested with TRYPSIN. The resulting peptide fragments were separated by chromatography. One of the peptide fragments isolated from the mixture is the peptide fragment Asp-Gly-Tyr-Leu-Val. Which statement below is TRUE about the location of this peptide fragment in the original protein? Possible Answers a. The peptide is released from the center of the protein b. The original protein has an N-terminal aspartic acid c. The original protein has a C-terminal valine d. None of the other choices are correct e. Insufficient information is given to answer 4. The Michaelis-Menten model for enzyme catalysis holds when: Possible Answers a. in the initial stages of reaction when [P] is negligible when [S] = [E] and under steady state conditions b. always c. under steady state conditions d. in the initial stages of reaction when [P] is negligible under steady state conditions and when [E] >> [S] e. in the initial stages of reaction when [P] is negligible under steady state conditions and when [E] 5. The following data are obtained in a study of an enzyme known to follow Michaelis-Menten kinetics given as pairs of data points in [] in the format: [Substrate added (mmol/L) Vo (mmol/min)]. Data: [0.8 2.2] [2 3.2] [4 4.3] [6 5.0] [20 6.5]. Consider a Michaelis-Menten plot of this data. The KM value for this enzyme IS APPROXIMATELY: Possible Answers a. 1 mM b. 2 mM c. 4 mM d. 6 mM e. 20 mM