BirdnestS22.pptx

    Nest predation: Developing a question and purpose:

    Example of a scientific question: Does nest height matter for influencing nest predation?

    Remember developing a question(hypothesis) for scientific research must meet the following requirements:

    Testable

    Repeatable

    Measurable; through statistical analysis

    Should be influenced by the scientific literature

    Should have a reason for testing; i.e., Why do we care, or why is it important to society, the environment, the public, etc.

    Tentative Location: (M,W, F classes)

    Flat Rock State Natural Area

    2381 Factory Road,

    Murfreesboro, TN 37130

    860 17’ 43”W, 350 51’ 30”N

    Analyzing Ecology:

    Independent variables: factors that are presumed to cause other variables to change.

    Dependent variables: factors that are being changed.

    Example:

    We hypothesize that variation in the nest condition has led to different predation rates.

    Independent variable = Nest condition

    Dependent variable = Predation

    * Experimental unit = nest

    5.5ft

    Use transects and marking them

    Map it or sketch it

    Assign treatment/control conditions along transects

    Random table generator or random # table

    Pattern

    Random

    Combination

    Layout experiment

    Brown Egg Nest

    Speckled Egg Nest

    POSITIVE SIGNS OF PREDATION

    For this class we focus on Chi Squared Statistical Method:

    “Goodness of Fit” Test

    2X2 Contingency Table analysis

    It is intended to test how likely it is that an observed distribution is due to chance. It is also called a "goodness of fit" statistic, because it measures how well the observed distribution of data fits with the distribution that is expected if the variables are independent.

    Data High Nest Low nest Total
    Predation 5 6 11
    No Predation 11 10 21
    Total 16 16 32

    Data Collection:

    Dependent Variable 

    Independent Variable 

    Pred/No Trt. Obs. Calc. Calc. Expected
    Pred high 5 Col1 x row1/Total (16 x 11)/32 5.5
    Pred low 6 Col2 x row1/total (16 x 11)/32 5.5
    No Pred high 11 Col1 x row2/total (16 x 21)/32 10.5
    No Pred low 10 Col2 x row2/total (16 x 21)/32 10.5

    How to calculate expected frequencies:

    Data High Nest Low nest Total
    Predation 5 6 11
    No Predation 11 10 21
    Total 16 16 32
    Pred/No Trt. Obs. Exp. (Obs – Exp)2 /Exp
    Pred High 5 5.5 (5 – 5.5)2/ 5.5 0.045
    Pred Low 6 5.5 (6 – 5.5)2/5.5 0.045
    No Pred High 11 10.5 (11 – 10.5)2/10.5 0.024
    No Pred Low 10 10.5 (10 – 10.5)2/10.5 0.024
    Sum of Chi-square 0.139

    Calculating Chi-square:

    From the observed and expected frequencies

    x2 = Σ(observed – expected)2

    expected

    x2 = (5-5.5) 2+ (11-10.5) 2+ (6-5.5) 2+ (10-10.5) 2

    5.5 10.5 5.5 10.5

    x2 = 0.139

    Hypothesis – Additional info and X2(Chi square):

    H0: The height of the nest has no effect on predation of eggs

    HA: The height of the nest does have an effect on predation of eggs

    Each group will have a X2(chi square critical value of 3.841), so if the calculated test statistic is…..

    ≥ critical value means that it supports the hypothesis of a difference (reject Ho)

    ≤ critical value means that it does NOT support the hypothesis of a difference (accept Ho))

    Interpretation

    Because our x2 is less than the critical value for p = 0.05, we fail to reject the null hypothesis

    There is no significant difference in the predation of nests placed high versus nests that low

    X2 = 0.139

    critical value (from table, p = 0.05) = 3.84

    0.139 < 3.84

    13

    Your Project:

    Model birds find at Flat Rock

    Lists on D2L

    Control & treatment

    Manipulate Nest or egg

    Shape, size, color, materials, or decoy

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